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pgex 6p 1 p37deltan  (Addgene inc)


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    Structured Review

    Addgene inc pgex 6p 1 p37deltan
    Pgex 6p 1 P37deltan, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgex+6p+1+p37deltan/pGEX-6P-1+p37deltaSEP+(Plasmid+%23113501)/pm30344098-267-108-112
    Average 90 stars, based on 1 article reviews
    pgex 6p 1 p37deltan - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Recombinant:

    Article Title: Ubiquitin-Independent Disassembly by a p97 AAA-ATPase Complex Drives PP1 Holoenzyme Formation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER DyLight 800 maleimide Thermo Fisher 46621 ATPgS Sigma A1388 Zeba Spin Desalting column Thermo Fisher 89882 Lipofectamine RNAiMAX transfection reagent Thermo Fisher 13778150 Recombinant p37 This study N/A Recombinant p37DSEP (D140-206) This study N/A Recombinant p37SHPmut (F215A/E218A/QKL220-222AAA) This study N/A Recombinant p37DN (D2-81) This study N/A Recombinant p37DUBX (D251-331) This study N/A Recombinant p37 F89pBpA This study N/A Recombinant p37 L182pBpA This study N/A Recombinant His-p97 This study N/A Recombinant p97-Strep-His E314pBpA This study N/A Recombinant p97-His D592pBpA This study N/A Recombinant His-SDS22-PP1g-I3 This study N/A Recombinant His-I3 This study N/A Recombinant His-I3 (V41A/W43A) This study N/A Recombinant SDS22-PP1g This study N/A Recombinant SDS22-PP1g-Eos-I3 This study N/A Recombinant His-NIPP1 Beullens et al., 2000 N/A Recombinant His-diUb-GFP Blythe et al., 2017 N/A Recombinant Ufd1-Npl4 Meyer et al., 2002 N/A Critical Commercial Assays CellTiter 96 AQueous One Solution Cell Proliferation Assay (MTS) Promega Cat. no. G3580 Deposited Data Original unprocessed gels, blots, and microscopy images used in this manuscript This study; Mendeley data https://doi.org/10.17632/ hfsb92kdh5.1 Mass spectrometry label-free quantification data: PP1g-specific interactors in wild-type HeLa cells versus p47 knockout cells with additional depletion of p37 and UBXN2A This study; PRIDE repository dataset identifier PXD010750 https://www.ebi.ac.uk/pride/ archive/ Experimental Models: Cell Lines HEK293 FlpIn T-REx p97 (wt)-myc/strep Ritz et al., 2011 N/A HEK293 FlpIn T-REx p97 (E578Q)-myc/strep Ritz et al., 2011 N/A HEK293 FlpIn T-REx p47-strep-HA This study N/A HEK293 FlpIn T-REx p37-strep-HA This study N/A HEK293 FlpIn T-REx UBXN2A-strep-HA This study N/A HEK293 FlpIn T-REx UBXN11-strep-HA This study N/A HEK293 FlpIn T-REx Ufd1-strep-HA This study N/A HeLa Kyoto RRID CVCL_1922 HeLa FlpIn T-REx GFP-strep-HA This study N/A HeLa FlpIn T-REx PP1g-strep This study N/A HeLa p47 knockout This study N/A Sf9 Invitrogen 11496015 Oligonucleotides siInhibitor-3 s1: CUGCUGTAUUUAUGAGAAATT Microsynth N/A siInhibitor-3 s2: GUAGAAUGGACAAGUGACATT Microsynth N/A sip97 s1: GGAGUUCAAAGUGGUGGAAACAGAUTT Microsynth N/A sip97 s2: AAGUAGGGUAUGAUGACAUUGTT Microsynth N/A (Continued on next page) e2 Molecular Cell 72, 1–12.e1–e6, November 15, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER sip47 s1: AGCCAGCUCUUCCAUCUUATT Microsynth N/A sip37 s1: GUGCCGUAAUAUAGAGGAATT Microsynth N/A sip37 s2: CAGUUUAGAUGAUGGAGAATT Microsynth N/A siUBXN2A s1: AGAAGAGGUGGACGUUAAATT Microsynth N/A siUBXN2A s2: GAAAUAUGUUUGUCUACGATT Microsynth N/A siPP1 Santa Cruz sc-43545 Recombinant DNA p47 CRISPR/Cas9 KO plasmid Santa Cruz sc-402328 p47 HDR plasmid Santa Cruz sc-402328-HDR pEVOL-pBpF plasmid Addgene #31190 pcDNA5FRT/TO-p37-Strep-HA H€ulsmann et al., 2018; Addgene #113485 pcDNA5FRT/TO-p47-Strep-HA H€ulsmann et al., 2018; Addgene #113475 pcDNA5FRT/TO-UBXN2A-Strep-HA H€ulsmann et al., 2018; Addgene #113480 pcDNA5FRT/TO-UBXN11-Strep-HA H€ulsmann et al., 2018; Addgene #113493 pcDNA5FRT/TO-Ufd1-Strep-HA H€ulsmann et al., 2018; Addgene #113474 pcDNA5/FRT/TO/GFP-SH R. Aebersold; H€ulsmann et al., 2018 N/A pGEX-6P-1 p37 This study; Addgene #113500 pGEX-6P-1 p37deltaSEP This study; Addgene #113501 pGEX-6P-1 p37deltaN This study; Addgene #113502 pGEX-6P-1 p37 SHPmut This study; Addgene #113503 pGEX-6P-1 p37 deltaUBX This study; Addgene #113504 pGEX-4T-1 UBXN2A This study; Addgene #113505 pcDNA5FRT/TO PP1gamma-2*Strep This study; Addgene #113506 pET15b His-p97 This study; Addgene #113507 pET15b p97-Strep-His E314Amb This study; Addgene #113508 pET15b p97-His D592Amb This study; Addgene #113509 pFL His-p97 This study; Addgene #113510 pFL His-SDS22 / PP1gamma This study; Addgene #113511 pFL His-I3 This study; Addgene #113513 pFL I3 This study; Addgene #113514 pFL His-I3 V41A / W43A This study; Addgene #113515 pFL His-mEos3.2-I3 This study; Addgene #113516 Software and Algorithms FlowJo v10.5.0 FlowJo, LLC https://www.flowjo.com/ OriginPro 2016G OriginLab https://www.originlab.com/ MaxQuant v1.5.3.30 and the MaxLFQ algorithm Cox et al., 2014 http://www.biochem.mpg.de/ 5111795/maxquant Perseus v1.5.5.3 Tyanova et al., 2016 http://www.biochem.mpg.de/ 5111810/perseus SigmaPlot v12.5 Systat Software http://www.systat.de/ SigmaPlot_Produktseite.html PyMol Schrödinger, LLC https://www.pymol.org/2/ Cell Profiler Kamentsky et al., 2011 http://www.cellprofiler.org/ .. Further information and requests for resources and reagents should be directed to and will be fulfilled by the Lead Contact, Hemmo Meyer (hemmo.meyer@uni-due.de).

    CRISPR:

    Article Title: Ubiquitin-Independent Disassembly by a p97 AAA-ATPase Complex Drives PP1 Holoenzyme Formation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER DyLight 800 maleimide Thermo Fisher 46621 ATPgS Sigma A1388 Zeba Spin Desalting column Thermo Fisher 89882 Lipofectamine RNAiMAX transfection reagent Thermo Fisher 13778150 Recombinant p37 This study N/A Recombinant p37DSEP (D140-206) This study N/A Recombinant p37SHPmut (F215A/E218A/QKL220-222AAA) This study N/A Recombinant p37DN (D2-81) This study N/A Recombinant p37DUBX (D251-331) This study N/A Recombinant p37 F89pBpA This study N/A Recombinant p37 L182pBpA This study N/A Recombinant His-p97 This study N/A Recombinant p97-Strep-His E314pBpA This study N/A Recombinant p97-His D592pBpA This study N/A Recombinant His-SDS22-PP1g-I3 This study N/A Recombinant His-I3 This study N/A Recombinant His-I3 (V41A/W43A) This study N/A Recombinant SDS22-PP1g This study N/A Recombinant SDS22-PP1g-Eos-I3 This study N/A Recombinant His-NIPP1 Beullens et al., 2000 N/A Recombinant His-diUb-GFP Blythe et al., 2017 N/A Recombinant Ufd1-Npl4 Meyer et al., 2002 N/A Critical Commercial Assays CellTiter 96 AQueous One Solution Cell Proliferation Assay (MTS) Promega Cat. no. G3580 Deposited Data Original unprocessed gels, blots, and microscopy images used in this manuscript This study; Mendeley data https://doi.org/10.17632/ hfsb92kdh5.1 Mass spectrometry label-free quantification data: PP1g-specific interactors in wild-type HeLa cells versus p47 knockout cells with additional depletion of p37 and UBXN2A This study; PRIDE repository dataset identifier PXD010750 https://www.ebi.ac.uk/pride/ archive/ Experimental Models: Cell Lines HEK293 FlpIn T-REx p97 (wt)-myc/strep Ritz et al., 2011 N/A HEK293 FlpIn T-REx p97 (E578Q)-myc/strep Ritz et al., 2011 N/A HEK293 FlpIn T-REx p47-strep-HA This study N/A HEK293 FlpIn T-REx p37-strep-HA This study N/A HEK293 FlpIn T-REx UBXN2A-strep-HA This study N/A HEK293 FlpIn T-REx UBXN11-strep-HA This study N/A HEK293 FlpIn T-REx Ufd1-strep-HA This study N/A HeLa Kyoto RRID CVCL_1922 HeLa FlpIn T-REx GFP-strep-HA This study N/A HeLa FlpIn T-REx PP1g-strep This study N/A HeLa p47 knockout This study N/A Sf9 Invitrogen 11496015 Oligonucleotides siInhibitor-3 s1: CUGCUGTAUUUAUGAGAAATT Microsynth N/A siInhibitor-3 s2: GUAGAAUGGACAAGUGACATT Microsynth N/A sip97 s1: GGAGUUCAAAGUGGUGGAAACAGAUTT Microsynth N/A sip97 s2: AAGUAGGGUAUGAUGACAUUGTT Microsynth N/A (Continued on next page) e2 Molecular Cell 72, 1–12.e1–e6, November 15, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER sip47 s1: AGCCAGCUCUUCCAUCUUATT Microsynth N/A sip37 s1: GUGCCGUAAUAUAGAGGAATT Microsynth N/A sip37 s2: CAGUUUAGAUGAUGGAGAATT Microsynth N/A siUBXN2A s1: AGAAGAGGUGGACGUUAAATT Microsynth N/A siUBXN2A s2: GAAAUAUGUUUGUCUACGATT Microsynth N/A siPP1 Santa Cruz sc-43545 Recombinant DNA p47 CRISPR/Cas9 KO plasmid Santa Cruz sc-402328 p47 HDR plasmid Santa Cruz sc-402328-HDR pEVOL-pBpF plasmid Addgene #31190 pcDNA5FRT/TO-p37-Strep-HA H€ulsmann et al., 2018; Addgene #113485 pcDNA5FRT/TO-p47-Strep-HA H€ulsmann et al., 2018; Addgene #113475 pcDNA5FRT/TO-UBXN2A-Strep-HA H€ulsmann et al., 2018; Addgene #113480 pcDNA5FRT/TO-UBXN11-Strep-HA H€ulsmann et al., 2018; Addgene #113493 pcDNA5FRT/TO-Ufd1-Strep-HA H€ulsmann et al., 2018; Addgene #113474 pcDNA5/FRT/TO/GFP-SH R. Aebersold; H€ulsmann et al., 2018 N/A pGEX-6P-1 p37 This study; Addgene #113500 pGEX-6P-1 p37deltaSEP This study; Addgene #113501 pGEX-6P-1 p37deltaN This study; Addgene #113502 pGEX-6P-1 p37 SHPmut This study; Addgene #113503 pGEX-6P-1 p37 deltaUBX This study; Addgene #113504 pGEX-4T-1 UBXN2A This study; Addgene #113505 pcDNA5FRT/TO PP1gamma-2*Strep This study; Addgene #113506 pET15b His-p97 This study; Addgene #113507 pET15b p97-Strep-His E314Amb This study; Addgene #113508 pET15b p97-His D592Amb This study; Addgene #113509 pFL His-p97 This study; Addgene #113510 pFL His-SDS22 / PP1gamma This study; Addgene #113511 pFL His-I3 This study; Addgene #113513 pFL I3 This study; Addgene #113514 pFL His-I3 V41A / W43A This study; Addgene #113515 pFL His-mEos3.2-I3 This study; Addgene #113516 Software and Algorithms FlowJo v10.5.0 FlowJo, LLC https://www.flowjo.com/ OriginPro 2016G OriginLab https://www.originlab.com/ MaxQuant v1.5.3.30 and the MaxLFQ algorithm Cox et al., 2014 http://www.biochem.mpg.de/ 5111795/maxquant Perseus v1.5.5.3 Tyanova et al., 2016 http://www.biochem.mpg.de/ 5111810/perseus SigmaPlot v12.5 Systat Software http://www.systat.de/ SigmaPlot_Produktseite.html PyMol Schrödinger, LLC https://www.pymol.org/2/ Cell Profiler Kamentsky et al., 2011 http://www.cellprofiler.org/ .. Further information and requests for resources and reagents should be directed to and will be fulfilled by the Lead Contact, Hemmo Meyer (hemmo.meyer@uni-due.de).

    Plasmid Preparation:

    Article Title: Ubiquitin-Independent Disassembly by a p97 AAA-ATPase Complex Drives PP1 Holoenzyme Formation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER DyLight 800 maleimide Thermo Fisher 46621 ATPgS Sigma A1388 Zeba Spin Desalting column Thermo Fisher 89882 Lipofectamine RNAiMAX transfection reagent Thermo Fisher 13778150 Recombinant p37 This study N/A Recombinant p37DSEP (D140-206) This study N/A Recombinant p37SHPmut (F215A/E218A/QKL220-222AAA) This study N/A Recombinant p37DN (D2-81) This study N/A Recombinant p37DUBX (D251-331) This study N/A Recombinant p37 F89pBpA This study N/A Recombinant p37 L182pBpA This study N/A Recombinant His-p97 This study N/A Recombinant p97-Strep-His E314pBpA This study N/A Recombinant p97-His D592pBpA This study N/A Recombinant His-SDS22-PP1g-I3 This study N/A Recombinant His-I3 This study N/A Recombinant His-I3 (V41A/W43A) This study N/A Recombinant SDS22-PP1g This study N/A Recombinant SDS22-PP1g-Eos-I3 This study N/A Recombinant His-NIPP1 Beullens et al., 2000 N/A Recombinant His-diUb-GFP Blythe et al., 2017 N/A Recombinant Ufd1-Npl4 Meyer et al., 2002 N/A Critical Commercial Assays CellTiter 96 AQueous One Solution Cell Proliferation Assay (MTS) Promega Cat. no. G3580 Deposited Data Original unprocessed gels, blots, and microscopy images used in this manuscript This study; Mendeley data https://doi.org/10.17632/ hfsb92kdh5.1 Mass spectrometry label-free quantification data: PP1g-specific interactors in wild-type HeLa cells versus p47 knockout cells with additional depletion of p37 and UBXN2A This study; PRIDE repository dataset identifier PXD010750 https://www.ebi.ac.uk/pride/ archive/ Experimental Models: Cell Lines HEK293 FlpIn T-REx p97 (wt)-myc/strep Ritz et al., 2011 N/A HEK293 FlpIn T-REx p97 (E578Q)-myc/strep Ritz et al., 2011 N/A HEK293 FlpIn T-REx p47-strep-HA This study N/A HEK293 FlpIn T-REx p37-strep-HA This study N/A HEK293 FlpIn T-REx UBXN2A-strep-HA This study N/A HEK293 FlpIn T-REx UBXN11-strep-HA This study N/A HEK293 FlpIn T-REx Ufd1-strep-HA This study N/A HeLa Kyoto RRID CVCL_1922 HeLa FlpIn T-REx GFP-strep-HA This study N/A HeLa FlpIn T-REx PP1g-strep This study N/A HeLa p47 knockout This study N/A Sf9 Invitrogen 11496015 Oligonucleotides siInhibitor-3 s1: CUGCUGTAUUUAUGAGAAATT Microsynth N/A siInhibitor-3 s2: GUAGAAUGGACAAGUGACATT Microsynth N/A sip97 s1: GGAGUUCAAAGUGGUGGAAACAGAUTT Microsynth N/A sip97 s2: AAGUAGGGUAUGAUGACAUUGTT Microsynth N/A (Continued on next page) e2 Molecular Cell 72, 1–12.e1–e6, November 15, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER sip47 s1: AGCCAGCUCUUCCAUCUUATT Microsynth N/A sip37 s1: GUGCCGUAAUAUAGAGGAATT Microsynth N/A sip37 s2: CAGUUUAGAUGAUGGAGAATT Microsynth N/A siUBXN2A s1: AGAAGAGGUGGACGUUAAATT Microsynth N/A siUBXN2A s2: GAAAUAUGUUUGUCUACGATT Microsynth N/A siPP1 Santa Cruz sc-43545 Recombinant DNA p47 CRISPR/Cas9 KO plasmid Santa Cruz sc-402328 p47 HDR plasmid Santa Cruz sc-402328-HDR pEVOL-pBpF plasmid Addgene #31190 pcDNA5FRT/TO-p37-Strep-HA H€ulsmann et al., 2018; Addgene #113485 pcDNA5FRT/TO-p47-Strep-HA H€ulsmann et al., 2018; Addgene #113475 pcDNA5FRT/TO-UBXN2A-Strep-HA H€ulsmann et al., 2018; Addgene #113480 pcDNA5FRT/TO-UBXN11-Strep-HA H€ulsmann et al., 2018; Addgene #113493 pcDNA5FRT/TO-Ufd1-Strep-HA H€ulsmann et al., 2018; Addgene #113474 pcDNA5/FRT/TO/GFP-SH R. Aebersold; H€ulsmann et al., 2018 N/A pGEX-6P-1 p37 This study; Addgene #113500 pGEX-6P-1 p37deltaSEP This study; Addgene #113501 pGEX-6P-1 p37deltaN This study; Addgene #113502 pGEX-6P-1 p37 SHPmut This study; Addgene #113503 pGEX-6P-1 p37 deltaUBX This study; Addgene #113504 pGEX-4T-1 UBXN2A This study; Addgene #113505 pcDNA5FRT/TO PP1gamma-2*Strep This study; Addgene #113506 pET15b His-p97 This study; Addgene #113507 pET15b p97-Strep-His E314Amb This study; Addgene #113508 pET15b p97-His D592Amb This study; Addgene #113509 pFL His-p97 This study; Addgene #113510 pFL His-SDS22 / PP1gamma This study; Addgene #113511 pFL His-I3 This study; Addgene #113513 pFL I3 This study; Addgene #113514 pFL His-I3 V41A / W43A This study; Addgene #113515 pFL His-mEos3.2-I3 This study; Addgene #113516 Software and Algorithms FlowJo v10.5.0 FlowJo, LLC https://www.flowjo.com/ OriginPro 2016G OriginLab https://www.originlab.com/ MaxQuant v1.5.3.30 and the MaxLFQ algorithm Cox et al., 2014 http://www.biochem.mpg.de/ 5111795/maxquant Perseus v1.5.5.3 Tyanova et al., 2016 http://www.biochem.mpg.de/ 5111810/perseus SigmaPlot v12.5 Systat Software http://www.systat.de/ SigmaPlot_Produktseite.html PyMol Schrödinger, LLC https://www.pymol.org/2/ Cell Profiler Kamentsky et al., 2011 http://www.cellprofiler.org/ .. Further information and requests for resources and reagents should be directed to and will be fulfilled by the Lead Contact, Hemmo Meyer (hemmo.meyer@uni-due.de).

    Software:

    Article Title: Ubiquitin-Independent Disassembly by a p97 AAA-ATPase Complex Drives PP1 Holoenzyme Formation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER DyLight 800 maleimide Thermo Fisher 46621 ATPgS Sigma A1388 Zeba Spin Desalting column Thermo Fisher 89882 Lipofectamine RNAiMAX transfection reagent Thermo Fisher 13778150 Recombinant p37 This study N/A Recombinant p37DSEP (D140-206) This study N/A Recombinant p37SHPmut (F215A/E218A/QKL220-222AAA) This study N/A Recombinant p37DN (D2-81) This study N/A Recombinant p37DUBX (D251-331) This study N/A Recombinant p37 F89pBpA This study N/A Recombinant p37 L182pBpA This study N/A Recombinant His-p97 This study N/A Recombinant p97-Strep-His E314pBpA This study N/A Recombinant p97-His D592pBpA This study N/A Recombinant His-SDS22-PP1g-I3 This study N/A Recombinant His-I3 This study N/A Recombinant His-I3 (V41A/W43A) This study N/A Recombinant SDS22-PP1g This study N/A Recombinant SDS22-PP1g-Eos-I3 This study N/A Recombinant His-NIPP1 Beullens et al., 2000 N/A Recombinant His-diUb-GFP Blythe et al., 2017 N/A Recombinant Ufd1-Npl4 Meyer et al., 2002 N/A Critical Commercial Assays CellTiter 96 AQueous One Solution Cell Proliferation Assay (MTS) Promega Cat. no. G3580 Deposited Data Original unprocessed gels, blots, and microscopy images used in this manuscript This study; Mendeley data https://doi.org/10.17632/ hfsb92kdh5.1 Mass spectrometry label-free quantification data: PP1g-specific interactors in wild-type HeLa cells versus p47 knockout cells with additional depletion of p37 and UBXN2A This study; PRIDE repository dataset identifier PXD010750 https://www.ebi.ac.uk/pride/ archive/ Experimental Models: Cell Lines HEK293 FlpIn T-REx p97 (wt)-myc/strep Ritz et al., 2011 N/A HEK293 FlpIn T-REx p97 (E578Q)-myc/strep Ritz et al., 2011 N/A HEK293 FlpIn T-REx p47-strep-HA This study N/A HEK293 FlpIn T-REx p37-strep-HA This study N/A HEK293 FlpIn T-REx UBXN2A-strep-HA This study N/A HEK293 FlpIn T-REx UBXN11-strep-HA This study N/A HEK293 FlpIn T-REx Ufd1-strep-HA This study N/A HeLa Kyoto RRID CVCL_1922 HeLa FlpIn T-REx GFP-strep-HA This study N/A HeLa FlpIn T-REx PP1g-strep This study N/A HeLa p47 knockout This study N/A Sf9 Invitrogen 11496015 Oligonucleotides siInhibitor-3 s1: CUGCUGTAUUUAUGAGAAATT Microsynth N/A siInhibitor-3 s2: GUAGAAUGGACAAGUGACATT Microsynth N/A sip97 s1: GGAGUUCAAAGUGGUGGAAACAGAUTT Microsynth N/A sip97 s2: AAGUAGGGUAUGAUGACAUUGTT Microsynth N/A (Continued on next page) e2 Molecular Cell 72, 1–12.e1–e6, November 15, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER sip47 s1: AGCCAGCUCUUCCAUCUUATT Microsynth N/A sip37 s1: GUGCCGUAAUAUAGAGGAATT Microsynth N/A sip37 s2: CAGUUUAGAUGAUGGAGAATT Microsynth N/A siUBXN2A s1: AGAAGAGGUGGACGUUAAATT Microsynth N/A siUBXN2A s2: GAAAUAUGUUUGUCUACGATT Microsynth N/A siPP1 Santa Cruz sc-43545 Recombinant DNA p47 CRISPR/Cas9 KO plasmid Santa Cruz sc-402328 p47 HDR plasmid Santa Cruz sc-402328-HDR pEVOL-pBpF plasmid Addgene #31190 pcDNA5FRT/TO-p37-Strep-HA H€ulsmann et al., 2018; Addgene #113485 pcDNA5FRT/TO-p47-Strep-HA H€ulsmann et al., 2018; Addgene #113475 pcDNA5FRT/TO-UBXN2A-Strep-HA H€ulsmann et al., 2018; Addgene #113480 pcDNA5FRT/TO-UBXN11-Strep-HA H€ulsmann et al., 2018; Addgene #113493 pcDNA5FRT/TO-Ufd1-Strep-HA H€ulsmann et al., 2018; Addgene #113474 pcDNA5/FRT/TO/GFP-SH R. Aebersold; H€ulsmann et al., 2018 N/A pGEX-6P-1 p37 This study; Addgene #113500 pGEX-6P-1 p37deltaSEP This study; Addgene #113501 pGEX-6P-1 p37deltaN This study; Addgene #113502 pGEX-6P-1 p37 SHPmut This study; Addgene #113503 pGEX-6P-1 p37 deltaUBX This study; Addgene #113504 pGEX-4T-1 UBXN2A This study; Addgene #113505 pcDNA5FRT/TO PP1gamma-2*Strep This study; Addgene #113506 pET15b His-p97 This study; Addgene #113507 pET15b p97-Strep-His E314Amb This study; Addgene #113508 pET15b p97-His D592Amb This study; Addgene #113509 pFL His-p97 This study; Addgene #113510 pFL His-SDS22 / PP1gamma This study; Addgene #113511 pFL His-I3 This study; Addgene #113513 pFL I3 This study; Addgene #113514 pFL His-I3 V41A / W43A This study; Addgene #113515 pFL His-mEos3.2-I3 This study; Addgene #113516 Software and Algorithms FlowJo v10.5.0 FlowJo, LLC https://www.flowjo.com/ OriginPro 2016G OriginLab https://www.originlab.com/ MaxQuant v1.5.3.30 and the MaxLFQ algorithm Cox et al., 2014 http://www.biochem.mpg.de/ 5111795/maxquant Perseus v1.5.5.3 Tyanova et al., 2016 http://www.biochem.mpg.de/ 5111810/perseus SigmaPlot v12.5 Systat Software http://www.systat.de/ SigmaPlot_Produktseite.html PyMol Schrödinger, LLC https://www.pymol.org/2/ Cell Profiler Kamentsky et al., 2011 http://www.cellprofiler.org/ .. Further information and requests for resources and reagents should be directed to and will be fulfilled by the Lead Contact, Hemmo Meyer (hemmo.meyer@uni-due.de).



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